| First Author | Ito A | Year | 1997 |
| Journal | J Autoimmun | Volume | 10 |
| Issue | 4 | Pages | 331-8 |
| PubMed ID | 9237796 | Mgi Jnum | J:42339 |
| Mgi Id | MGI:1095618 | Doi | 10.1006/jaut.1997.0142 |
| Citation | Ito A, et al. (1997) Regulation of autoimmune diabetes by interleukin 3-dependent bone marrow-derived cells in NOD mice. J Autoimmun 10(4):331-8 |
| abstractText | Interleukin-3 (IL-3), a multilineage colony stimulating factor, has been shown to augment alloreactive bone marrow-derived suppressor cell activity in vivo and in vitro. The present study examined the effect of IL-3 on autoimmune-mediated diabetes in NOD mice. Administration of IL-3 twice weekly starting at 2-4 weeks of age delayed the onset and reduced the overall incidence of diabetes. Bone marrow cells obtained from IL-3-treated NOD mice protected NOD mice from cyclophosphamide-induced diabetes but failed to prevent adoptively transferred diabetes. In vitro culture of bone marrow cells in medium containing IL-3 produced a Thy-1+CD3epsilonloCD4-CD8-CD25- immature T cell clone which prevented cyclophosphamide-induced diabetes. The cloned cells also effectively delayed the development of diabetes induced by transfer of T cells in adult thymectomized, irradiated, bone marrow-reconstituted NOD mice. These results suggest that IL-3 is capable of regulating extrathymic T cell development from the bone marrow and that these cells mediate strong immunoregulatory function. |
