| First Author | Herrero MB | Year | 1997 |
| Journal | FEBS Lett | Volume | 411 |
| Issue | 1 | Pages | 39-42 |
| PubMed ID | 9247138 | Mgi Jnum | J:41693 |
| Mgi Id | MGI:894223 | Doi | 10.1016/s0014-5793(97)00570-x |
| Citation | Herrero MB, et al. (1997) The nitric oxide synthase of mouse spermatozoa. FEBS Lett 411(1):39-42 |
| abstractText | Nitric oxide synthase (NOS) was evidenced in mature mouse spermatozoa by means of biochemical techniques and Western blot. During 120 min of incubation, 10(7) spermatozoa synthesized 7 +/- 2 pmol of L-[14C]citrulline. Besides, L-citrulline formation depended on the incubation time and on the concentration of L-arginine present in the incubation medium. Different concentrations of N(G)-nitro-L-arginine methyl ester (L-NAME) but not aminoguanidine, inhibited L-[14C]citrulline formation. Western-blot analysis of solubilized sperm proteins revealed a unique band of M(r)=140 kDa with the neural, endothelial and inducible NOS antisera tested. These results provide evidence that mature mouse sperm contains a NOS isoform and that spermatozoa have the potential ability to synthesize NO, suggesting a role for endogenous NO on mammalian sperm function. |
