| First Author | Schmidt C | Year | 1997 |
| Journal | Dev Biol | Volume | 189 |
| Issue | 2 | Pages | 161-73 |
| PubMed ID | 9299111 | Mgi Jnum | J:43170 |
| Mgi Id | MGI:1097270 | Doi | 10.1006/dbio.1997.8661 |
| Citation | Schmidt C, et al. (1997) T promoter activity in the absence of functional T protein during axis formation and elongation in the mouse. Dev Biol 189(2):161-73 |
| abstractText | The T (Brachyury) gene, which encodes a transcription factor, is involved in the formation of posterior mesoderm. Although studies in Xenopus have shown that T gene expression is responsive to mesoderm inducing factors and furthermore suggest the existence of an autoregulatory loop involving T itself, eFGF, and the FGF receptor, little is known about the regulation of T expression in the mouse. We report here that in the mouse the expression of fgf-4, the putative homologue of Xenopus efgf, is not dependent on the presence of T protein during the first 48 hr of gastrulation. Furthermore, we address the question of autoregulation using a chimeric approach. Introduction of a T promoter-lacZ construct into T/T ES cells results in T promoter activity in the primitive streak and tail bud in the absence of functional T protein. Therefore, we suggest that a direct FGF and T autoregulatory loop is unlikely to operate during gastrulation and axis elongation in the mouse. Copyright 1997 Academic Press. |
