| First Author | Ye X | Year | 1997 |
| Journal | Biochem Biophys Res Commun | Volume | 239 |
| Issue | 3 | Pages | 660-5 |
| PubMed ID | 9367824 | Mgi Jnum | J:44064 |
| Mgi Id | MGI:1099315 | Doi | 10.1006/bbrc.1997.7529 |
| Citation | Ye X, et al. (1997) Suppression of cytochrome P450 Cyp2f2 mRNA levels in mice by the peroxisome proliferator diethylhexylphthalate. Biochem Biophys Res Commun 239(3):660-5 |
| abstractText | Exposure to peroxisome proliferators, which are extensively used, causes a number of pleiotrophic effects. Prolonged exposure to the peroxisome proliferator, DEHP, causes hepatic hyperplasia and liver tumors in rats and mice. This exposure can also induce a number of enzymes. To identify additional genes that are regulated by DEHP, mRNA differential display was used. One of the genes affected is cytochrome 450 Cyp2f2, a naphthalene hydroxylase. Using northern analysis, RNase protection assay, and RT-PCR, we show that the Cyp2f2 mRNA levels are decreased in mouse liver following DEHP treatment. A smaller Cyp2f2 mRNA transcript was also detected in kidney and these transcript levels were also suppressed but to a lesser extent than that in the liver. The response to DEHP in mouse liver is both dose and time dependent. |
