| First Author | Okano F | Year | 1997 |
| Journal | J Interferon Cytokine Res | Volume | 17 |
| Issue | 11 | Pages | 713-8 |
| PubMed ID | 9402110 | Mgi Jnum | J:44669 |
| Mgi Id | MGI:1100795 | Doi | 10.1089/jir.1997.17.713 |
| Citation | Okano F, et al. (1997) Cloning and expression of the cDNA for canine interleukin-12. J Interferon Cytokine Res 17(11):713-8 |
| abstractText | We cloned the canine interleukin-12 (IL-12) subunit cDNA. Canine IL-12 exhibited sequence homology to the known sequences of human, mouse, and bovine genes at nucleotide and amino acid levels. Cotransfection of the p35 and p40 subunits of canine IL-12 cDNA clones into COS-1 cells resulted in the secretion of IL-12, which supported proliferation of the stimulated canine lymphocytes, promoted induction of canine interferon-gamma (IFN-gamma) from canine lymphocytes, and showed antitumor effect in vitro. The cloned canine IL-12 will be useful for canine therapeutic applications. |
