| First Author | Bläsius R | Year | 1998 |
| Journal | J Neurochem | Volume | 70 |
| Issue | 4 | Pages | 1357-65 |
| PubMed ID | 9523551 | Mgi Jnum | J:46571 |
| Mgi Id | MGI:1201320 | Doi | 10.1046/j.1471-4159.1998.70041357.x |
| Citation | Blasius R, et al. (1998) A novel orphan G protein-coupled receptor primarily expressed in the brain is localized on human chromosomal band 2q21. J Neurochem 70(4):1357-65 |
| abstractText | A human hippocampus cDNA library was screened with a probe obtained from degenerate RT-PCR aimed at P2Y-homologous sequences. A positive clone, designated hip4, was identified containing an open reading frame of 1,020 bp that had been previously detected in a published genomic clone called R12. Subsequent screening of a human fetal brain cDNA library yielded a splice variant with a 1,104-bp open reading frame, which was named fb1. Both variants display the seven-transmembrane topology that is typical for G protein-coupled receptors. Probing a human multitissue northern blot revealed two brain-specific transcripts of 2.3 and 6.3 kb, respectively. Northern blot analyses with specific fragments confirmed that the two transcripts are generated by alternative polyadenylation yielding two different 3' untranslated regions. A genomic clone from the corresponding gene was isolated and mapped to human chromosomal band 2q21 by fluorescence in situ hybridization. |
