| First Author | Ema H | Year | 1998 |
| Journal | Eur J Immunol | Volume | 28 |
| Issue | 5 | Pages | 1563-9 |
| PubMed ID | 9603461 | Mgi Jnum | J:47631 |
| Mgi Id | MGI:1203871 | Doi | 10.1002/(SICI)1521-4141(199805)28:05<1563::AID-IMMU1563>3.0.CO;2-R |
| Citation | Ema H, et al. (1998) Development of T cell precursor activity in the murine fetal liver. Eur J Immunol 28(5):1563-9 |
| abstractText | The generation of T cell precursors in the liver of murine embryos was studied. The total number of T cell precursors in the liver was measured in thymic organ cultures by a limiting dilution assay. Sixty T cell precursors were detected in the liver at day 11 of gestation. By day 12 the number of precursors showed a 20-fold increase, half of which could be explained by in situ proliferation as ascertained by a fetal liver organ culture assay. By day 13 a further 2-3-fold increase was observed. Whereas the number of total liver cells continued to increase, that of T cell precursors declined in the following days, suggesting a massive exit of these cells after day 13. The capacity to generate a TCRB repertoire in the cells was evaluated by a PCR assay. T cell precursors in day 11 fetal liver developed a TCRB repertoire at day 8 of culture. The cells from days 12-15 developed an identically diverse repertoire by day 6, suggesting that day 11 precursors are more immature than those of later days. A mechanism for yielding a single wave of T cell precursors in the fetal liver is discussed with a proposed model. |
