| First Author | Fleming IN | Year | 1998 |
| Journal | FEBS Lett | Volume | 429 |
| Issue | 3 | Pages | 229-33 |
| PubMed ID | 9662423 | Mgi Jnum | J:48454 |
| Mgi Id | MGI:1270019 | Doi | 10.1016/s0014-5793(98)00566-3 |
| Citation | Fleming IN, et al. (1998) Phospholipase C-gamma, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in platelet-derived growth factor-induced phosphorylation of Tiam1. FEBS Lett 429(3):229-33 |
| abstractText | In Swiss 3T3 fibroblasts, the Rac1-specific guanine nucleotide exchange factor Tiam1 is phosphorylated by several different agonists. We show here that PDGF induces threonine phosphorylation of Tiam1 in a time-and dose-dependent manner. Tiam1 phosphorylation was significantly reduced by the selective protein kinase C inhibitor Ro-31-8220 and by KN93, an inhibitor of Ca2+/calmodulin-dependent protein kinase II. The Ca2+ chelator BAPTA/AM totally abrogated Tiam1 phosphorylation, indicating that Ca2+ is essential for this phosphorylation. Moreover, PDGF-stimulated Tiam1 phosphorylation was markedly reduced by 72 +/- 10% in PLC-gamma1 deficient mouse fibroblasts, compared to wild-type cells, indicating that phosphoinositide phospholipase C is involved. |
