| First Author | Kamata T | Year | 1998 |
| Journal | Brain Res Mol Brain Res | Volume | 57 |
| Issue | 2 | Pages | 201-10 |
| PubMed ID | 9675418 | Mgi Jnum | J:48524 |
| Mgi Id | MGI:1270094 | Doi | 10.1016/s0169-328x(98)00082-5 |
| Citation | Kamata T, et al. (1998) Xenopus CRMP-2 is an early response gene to neural induction. Brain Res Mol Brain Res 57(2):201-10 |
| abstractText | A neural specific protein, CRMP-2 (for Collapsin Response Mediator Protein-2), is considered to mediate collapsin-induced growth cone collapse during neural development. We have isolated the Xenopus homologue of the CRMP-2 (XCRMP-2) cDNA and studied the expression of XCRMP-2 mRNA and protein during neural induction. Induction of XCRMP-2 mRNA and protein expression, like N-CAM, occurred at the midgastrula stage and increased through early neural developmental stages. Whole mount in situ hybridization demonstrated that expression of XCRMP-2 mRNA was localized in neural tissues such as the neural plate and tube at early stages, while its expression in the brain, spinal cord, and eyes was observed at later stages. Immunostaining of Xenopus embryos with the antibody against CRMP-2 also showed that the protein was specifically expressed in the neural tissues at early stages. XCRMP-2 expression was induced by neural inducers such as noggin and chordin which antagonize a neural inhibitor, BMP4. A dominant negative BMP receptor also induced XCRMP-2 expression, suggesting that transcription of XCRMP-2 gene was negatively regulated by the BMP4 signaling. These results indicate that expression of XCRMP-2 is an early response marking neural commitment, and that transcriptional control of XCRMP-2 gene, is one of the targets of BMP4 signaling. |
