| First Author | Yahata T | Year | 1998 |
| Journal | Immunol Lett | Volume | 62 |
| Issue | 3 | Pages | 165-70 |
| PubMed ID | 9698115 | Mgi Jnum | J:48842 |
| Mgi Id | MGI:1275875 | Doi | 10.1016/s0165-2478(98)00044-3 |
| Citation | Yahata T, et al. (1998) Reconstitution of immune systems in RAG2-/- mice by transfer with interleukin-12-induced splenic hematopoietic progenitor cells. Immunol Lett 62(3):165-70 |
| abstractText | The administration of a high dose of IL-12 into the mice resulted in the induction of spleno-megaly. From the flow cytometry analysis of cellularity in an enlarged spleen, it was demonstrated that Thyl.2-CD45RB-c-Kit + Sca-1 + Lin- hematopoietic progenitor cells markedly increased in IL-12-administered mouse spleen compared with untreated mouse spleen. The IL-12-induced hematopoietic progenitor cells showed a greatly enhanced colony-forming activity in CFU-granulocyte/macrophage (CFU-GM), blast-forming units-erythroid (BFU-E) and CFU-spleen (CFU-S) assay. Moreover, it was initially demonstrated that the transfer of IL-12-induced splenic hematopoietic progenitor cells into immunodeficient RAG2-/- mice caused a complete reconstitution of their immune functions including T- and B-cell-mediated immunity. Thus, the evidence that IL-12 has a capability of inducing hematopoietic progenitor cells possessing stem cell-like activity in vivo, indicated another important immunomodulating activity of IL-12 in immunotherapy. |
