| First Author | Kobayashi M | Year | 1998 |
| Journal | Gene | Volume | 215 |
| Issue | 2 | Pages | 329-37 |
| PubMed ID | 9714832 | Mgi Jnum | J:49461 |
| Mgi Id | MGI:1277506 | Doi | 10.1016/s0378-1119(98)00283-2 |
| Citation | Kobayashi M, et al. (1998) Characterization of the 3' untranslated region of mouse DNA topoisomerase IIalpha mRNA. Gene 215(2):329-37 |
| abstractText | Expression of DNA topoisomerase II alpha protein varies through the cell cycle with its peak in G(2)/M. This cell- cycle-dependent expression depends on changes in topoisomerase II alpha mRNA stability as well as promoter activity. We isolated the 3' genomic region of the mouse topoisomerase II alpha gene and investigated whether or not the 3' untranslated region (UTR) of the topoisomerase II alpha mRNA participates in the cell-cycle-dependent mRNA stability. Interestingly, genomic- and RT-PCR analyses revealed that the topoisomerase IIa 3' UTR is formed via splicing in mouse, but not in human and hamster. Comparison of the mouse 3' region with the human and hamster regions suggests that this mouse-specific splicing has resulted from an accidental acquisition of the consensus 5' splice site. The minority of the non- spliced topoisomerase II alpha 3' UTR in mouse was confirmed by Northern blot analysis. We performed transient expression assays using luciferase constructs with the mouse topoisomerase II alpha 3' genomic region, or the major spliced form of the 3' UTR. However, neither construct affected the cell-cycle-dependent expression of the reporter gene driven by the topoisomerase II alpha promoter. Our results strongly suggest that the mouse topoisomerase II alpha 3' UTR by itself is not involved in the cell-cycle-dependent mRNA stability. (C) 1998 Elsevier Science B.V. All rights reserved. |
