| First Author | Iouzalen N | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 250 |
| Issue | 2 | Pages | 359-63 |
| PubMed ID | 9753634 | Mgi Jnum | J:50045 |
| Mgi Id | MGI:1289780 | Doi | 10.1006/bbrc.1998.9336 |
| Citation | Iouzalen N, et al. (1998) Identification and characterization in Xenopus of XsmgGDS, a RalB binding protein. Biochem Biophys Res Commun 250(2):359-63 |
| abstractText | We have previously isolated a cDNA coding for a RalB protein from a Xenopus maternal library. By mutagenesis of RalB and microinjection of its mRNA in embryos we show that RalB is involved in Xenopus early development. We have used the yeast two-hybrid system to screen a Xenopus maternal cDNA library in order to isolate proteins interacting to RalB. We have identified a full-length cDNA encoding a protein homologous to mammalian smgGDS. The Xenopus gene shows 84.6% identity with the mammalian counterpart and contains one additional armadillo repeat. The XsmgGDS mRNA is expressed from oogenesis up to late embryogenesis at a higher level than that in adult tissues. Thus RalB is another protein that interacts with smgGDS which suggests that RalB may be activated by a Ras independent pathway. |
