| First Author | D'Andréa S | Year | 1998 |
| Journal | Mol Reprod Dev | Volume | 51 |
| Issue | 3 | Pages | 225-34 |
| PubMed ID | 9771642 | Mgi Jnum | J:50225 |
| Mgi Id | MGI:1290046 | Doi | 10.1002/(SICI)1098-2795(199811)51:3<225::AID-MRD1>3.0.CO;2-R |
| Citation | D'Andrea S, et al. (1998) Cloning of the porcine interferon-gamma receptor and its foeto-endometrial expression in early pregnancy. Mol Reprod Dev 51(3):225-34 |
| abstractText | In early gestation, trophoblastic cells of porcine preimplanting conceptuses transiently and massively secrete two distinct interferons (IFNs), one of which is IFN-gamma. In order to localize possible cellular target(s) for this IFN-gamma, the expression of the porcine IFN-gamma receptor and its developmental regulation have been investigated on the maternal endometrium and on the embryonic tissues. A cDNA encoding the porcine IFN-gamma binding-chain (pIFNGR1) was isolated. When expressed in COS-7 cells, it displayed a specific binding to radiolabelled pIFN-gamma and was shown to be a glycosylated membrane protein with an apparent molecular mass of 92 kDa. Porcine IFNGR1 mRNA was detected by RT-PCR not only in uterine epithelial cells but also in embryonic tissues from at least as early as day 10 of gestation. Moreover, membrane expression of the pIFN-gamma receptor quantified by binding and crosslinking of 32P-pIFN-gamma was demonstrated in uterine epithelium and in the trophoblast. In the trophoblast, expression of the receptor was found to be developmentally regulated: although expression was weak on days 12 and 15 of gestation, it reached a level similar to that found on some IFN-gamma-sensitive cells on day 16. This study shows that maternal endometrium is not the only possible target for trophoblastic IFN-gamma: the induction of pIFN- gamma receptor expression in the trophoblast around day 16 of gestation could suggest the appearance of responsiveness to pIFN-gamma in this implanted tissue and therefore a possible delayed autocrine effect of trophoblastic pIFN-gamma. |
