| First Author | Wubbel L | Year | 1998 |
| Journal | J Infect Dis | Volume | 178 |
| Issue | 5 | Pages | 1526-9 |
| PubMed ID | 9780280 | Mgi Jnum | J:50885 |
| Mgi Id | MGI:1313007 | Doi | 10.1086/314439 |
| Citation | Wubbel L, et al. (1998) Mycoplasma pneumoniae pneumonia in a mouse model. J Infect Dis 178(5):1526-9 |
| abstractText | To investigate the pathogenesis of acute Mycoplasma pneumoniae infection, BALB/c mice were anesthetized with metofane, and M. pneumoniae was introduced intranasally on days 0, 1, and 2. Mice were sacrificed on days 0-15. A histopathologic scoring system defined inflammatory changes in the lungs on a scale of 0-26 (least to most severe). Broth cultures were positive for all nasal passage and bronchoalveolar lavage (BAL) specimens. Histopathologic scores ranged from 0 to 21. The mean log10 (cfu/mL) were 4.1-6.4 on days 1-10 and >/=1.7 on days 13-15 for nasal passage and BAL specimens. Serum polymerase chain reaction was negative. ELISA for serum IgM and immunoblots for M. pneumoniae antibody were positive in 21 (62%) of 34 and 33 (97%) of 34 infected animals, respectively, at days 8-15. ELISA for IgG antibody was negative. This mouse pneumonia model can be used to study the immunologic and therapeutic responses to acute M. pneumoniae infection. |
