| First Author | Damario MA | Year | 1998 |
| Journal | J Assist Reprod Genet | Volume | 15 |
| Issue | 8 | Pages | 513-20 |
| PubMed ID | 9785199 | Mgi Jnum | J:49984 |
| Mgi Id | MGI:1289686 | Doi | 10.1023/A:1022538621928 |
| Citation | Damario MA, et al. (1998) Immunohistochemical analysis of insulin-like growth factor-binding proteins -1, -2, and -3 in implantation sites of the mouse. J Assist Reprod Genet 15(8):513-20 |
| abstractText | PURPOSE: Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor-binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse. METHODS: Six-week-old B6D2F1 female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin- embedded tissue was used for avidin-biotin immunocytochemical localization of IGFBPs utilizing standard methodology. RESULTS: Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5-10.5 days post coitum, there was moderate-dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua. CONCLUSIONS: Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals. |
