| First Author | Ni A | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1442 |
| Issue | 2-3 | Pages | 177-85 |
| PubMed ID | 9804950 | Mgi Jnum | J:50923 |
| Mgi Id | MGI:1313048 | Doi | 10.1016/s0167-4781(98)00163-8 |
| Citation | Ni A, et al. (1998) Molecular cloning and expression of rat bradykinin B1 receptor. Biochim Biophys Acta 1442(2-3):177-85 |
| abstractText | The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-kappaB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK>Lys-des-Arg9-BK approximately des-Arg9, Leu8-BK>Sar-Tyr-epsilonAhx-Lys-[D-betaNal7, Ile8]-des-Arg9-BK>Sar-Tyr-epsilonAhx-Lys-des-Arg9-BK>>BK>> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor. |
