| First Author | Sage J | Year | 1998 |
| Journal | Gene | Volume | 221 |
| Issue | 1 | Pages | 85-92 |
| PubMed ID | 9852953 | Mgi Jnum | J:51473 |
| Mgi Id | MGI:1316802 | Doi | 10.1016/s0378-1119(98)00420-x |
| Citation | Sage J, et al. (1998) Germ cell-specific enhancer activity of a repeated element in a variable region of the mouse genome. Gene 221(1):85-92 |
| abstractText | We recently described a complex genetic structure on mouse chromosome 8, a region of the murine genome in which genetic rearrangements frequently occur. A large repeated element specific to this chromosome was found to overlap with one of the cadherin genes (Cad11). An additional degree of complexity became apparent with the identification, in a subset of laboratory strains of mice, of a retrogene integrated into one of the repeated units. Designated Sycp1-ps2, it originated from the early meiotic gene encoding Synaptonemal Complex Protein 1. We now report that, among wild Mus species in which the retrogene is not present, this region of Chr 8 shows a high degree of variability. Sequence analysis showed that integration of Sycp1-ps2 created a 5' transcription initiator element. Transcription of the pseudogene in the testis was directly demonstrated. A germ cell-specific enhancer activity was localized within a 1117 bp region of the repeat, which was sufficient to direct the expression of reporter genes in transgenic mice to late meiotic and post-meiotic spermatogenic cells. |
