| First Author | Nugent P | Year | 1998 |
| Journal | In Vitro Cell Dev Biol Anim | Volume | 34 |
| Issue | 10 | Pages | 831-5 |
| PubMed ID | 9870533 | Mgi Jnum | J:54723 |
| Mgi Id | MGI:1335770 | Doi | 10.1007/s11626-998-0038-5 |
| Citation | Nugent P, et al. (1998) MSX-1 gene expression and regulation in embryonic palatal tissue. In Vitro Cell Dev Biol Anim 34(10):831-5 |
| abstractText | The palatal cleft seen in Msx-1 knock-out mice suggests a role for this gene in normal palate development. The cleft is presumed secondary to tooth and jaw malformations, since in situ hybrid-ization suggests that Msx-1 mRNA is not highly expressed in developing palatal tissue. In this study we demonstrate, by Northern blot analysis, the expression of Msx-1, but not Msx-2, in the develop-ing palate and in primary cultures of murine embryonic palate mesenchymal cells. Further-more, we propose a role for Msx-1 in retinoic acid-induced cleft palate, since retinoic acid inhibits Msx-1 mRNA expression in palate mesenchymal cells. We also demonstrate that transforming growth factor beta inhibits Msx-1 mRNA expression in palate mesenchymal cells, with retinoic acid and transform-ing growth factor beta acting synergistically when added simultaneously to these cells. These data suggest a mechanistic interaction between retinoic acid, transforming growth factor beta, and Msx-1 in the etiology of retinoic acid-induced cleft palate. |
