| First Author | Novobrantseva TI | Year | 1999 |
| Journal | J Exp Med | Volume | 189 |
| Issue | 1 | Pages | 75-88 |
| PubMed ID | 9874565 | Mgi Jnum | J:52061 |
| Mgi Id | MGI:1327761 | Doi | 10.1084/jem.189.1.75 |
| Citation | Novobrantseva TI, et al. (1999) Rearrangement and expression of immunoglobulin light chain genes can precede heavy chain expression during normal B cell development in mice. J Exp Med 189(1):75-88 |
| abstractText | In mouse mutants incapable of expressing mu chains, V(kappa)J(kappa) joints are detected in the CD43(+) B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) D cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive V(H)D(H)J(H) joint. Thus, mu, chain expression is not a prerequisite to Ig kappa light chain gene rearrangements in normal development. Overall, similar to 15% of the total CD43(+) B cell progenitor population carry Ig kappa gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43(+) progenitors rearrange IgH and Ig kappa loci independently, with a seven times higher frequency in the former. In addition, we show that ill B cell progenitors V(kappa)J(kappa) joining rapidly initiates kappa chain expression, irrespective of the presence of a mu chain. |
