| First Author | Leprini A | Year | 1998 |
| Journal | Cytogenet Cell Genet | Volume | 83 |
| Issue | 1-2 | Pages | 115-23 |
| PubMed ID | 9925948 | Mgi Jnum | J:53704 |
| Mgi Id | MGI:1333306 | Doi | 10.1159/000015146 |
| Citation | Leprini A, et al. (1998) Rat tenascin-R gene: structure, chromosome location and transcriptional activity of promoter and exon 1. Cytogenet Cell Genet 83(1-2):115-23 |
| abstractText | Tenascin-R is an extracellular matrix protein expressed exclusively in the central nervous system where it is thought to play a relevant role in regulating neurite outgrowth. We have i) cloned the cDNA of the rat tenascin-R 5' region; ii) defined its genomic organization, obtaining the sequence of two novel untranslated exons; iii) mapped the gene to rat chromosome 13q23 and suggested a previously unreported synteny between rat chromosome 13q23, human chromosome 1q24, and mouse chromosome 4E; and iv) sequenced and characterized the elements responsible for its neural cell-restricted transcription. We found that two discrete regions of the rat gene (the first in the proximal promoter, the second in the first exon) are independently able to activate to a high degree the transcription of a reporter gene in either human or rat neuroblastoma cell lines but not in other cell lines. Based on this observation, we re-evaluated the arrangement of transcriptionally active regions in the human tenascin-R gene we recently cloned and found that the human gene also contains an exon sequence able to initiate and sustain transcription independently of promoter sequences. |
