| First Author | Ever L | Year | 1999 |
| Journal | Cell Growth Differ | Volume | 10 |
| Issue | 1 | Pages | 19-26 |
| PubMed ID | 9950214 | Mgi Jnum | J:52045 |
| Mgi Id | MGI:1327736 | Citation | Ever L, et al. (1999) Two alternatively spliced Meig1 messenger RNA species are differentially expressed in the somatic and in the germ-cell compartments of the testis. Cell Growth Differ 10(1):19-26 |
| abstractText | Previous studies regarding the transcriptional pattern of the murine Meig1 gene (formally designated meg1) suggested that its transcription is restricted to germ cells at the first meiotic prophase, in both primary spermatocytes and primary oocytes. However, protein analysis revealed that certain forms of the MEIG1 protein exist in testes of early postnatal pups at stages that have no germ cells in the testis, excluding very few primitive type A spermatogonia cells. This suggested that MEIG1 expression is not confined to germ cells. In this study, we show that testicular somatic cells do, indeed, express MEIG1. This is especially evident in Leydig cells, where this protein is highly abundant. We also demonstrate that alternatively spliced mRNAs of Meig1 are differentially transcribed in the germ cell and the somatic compartments of the testis. There is a very low level of somatic transcript, whether labile or transcriptionally regulated, in contrast to the abundant MEIG1 protein in the somatic cells. This implies that the somatic transcript is very efficiently translated and reconfirms that protein levels do not necessarily reflect transcript abundancy. Structural features of the Meig1 transcript that would be expected to inhibit translation are discussed in light of the efficient translation of this RNA species. |
