| First Author | Rusciano D | Year | 1999 |
| Journal | J Cell Sci | Volume | 112 ( Pt 5) |
| Pages | 623-30 | PubMed ID | 9973597 |
| Mgi Jnum | J:53961 | Mgi Id | MGI:1333686 |
| Doi | 10.1242/jcs.112.5.623 | Citation | Rusciano D, et al. (1999) Regulation of c-met expression in B16 murine melanoma cells by melanocyte stimulating hormone. J Cell Sci 112(Pt 5):623-30 |
| abstractText | B16 murine melanoma cells selected in vivo for enhanced liver metastatic ability (B16-LS9) show on the one hand an increased expression and constitutive activation of the proto-oncogene c-met (the receptor for hepatocyte growth factor/scatter factor), and on the other hand a more differentiated phenotype, when compared to the parental cell line, B16-F1. Following this observation, we have tried to establish whether there is a direct relationship between differentiation and c-met expression in B16 melanoma cells. Treatment of these cells with differentiating agents indicated that c-met expression was strongly induced by melanocyte stimulating hormone, while retinoic acid had almost no influence. c-met induction was triggered by engagement of the melanocortin receptor, cAMP elevation and PKA/PKCalpha activation, as respectively shown by the effects of ACTH, cAMP elevating agents and specific PK inhibitors. Regulation of c-met expression via the melanocortin receptor and cAMP raises the intriguing possibility that autocrine and/or paracrine mechanisms acting in vivo on this circuit might influence (through c-met expression and activation) the metastatic behavior of these tumor cells, which we have shown to be dependent on their c-met expression. |
