| First Author | Sakai Y | Year | 1999 |
| Journal | Kidney Int | Volume | 55 |
| Issue | 3 | Pages | 833-40 |
| PubMed ID | 10027920 | Mgi Jnum | J:53787 |
| Mgi Id | MGI:1333412 | Doi | 10.1046/j.1523-1755.1999.055003833.x |
| Citation | Sakai Y, et al. (1999) The prolactin gene is expressed in the mouse kidney. Kidney Int 55(3):833-40 |
| abstractText | BACKGROUND: Prolactin (PRL), originally identified as an anterior pituitary hormone exhibiting lactogenic activity, is now recognized as a versatile hormone expressed in a wide variety of tissues. METHODS: In this study, the expression of PRL in the mouse kidney was investigated by solution-phase and in situ reverse transcription-polymerase chain reaction (RT-PCR) methods and immunohistochemistry. RESULTS: Mouse PRL (mPRL) transcript and protein are localized in the parietal epithelial cells of Bowman's capsule. Pit-1 is a positive transcription factor for the expression of the PRL gene. The presence of Pit-1 transcript in the kidney was also assessed by RT-PCR methods. The localization of Pit-1 mRNA coincided well with that of PRL. Immunoreactivity to mouse PRL receptor (mPRL-R) is distributed on the luminal membrane of the proximal tubule cells and the parietal epithelial cells of Bowman's capsule. CONCLUSION: These data indicate that the parietal epithelial cells of Bowman's capsule synthesize PRL de novo and suggest that Pit-1 contributes to the transcriptional regulation of PRL gene expression in the kidney, and PRL expressed in this tissue functions in an autocrine/paracrine fashion. |
