| First Author | Mohr U | Year | 1999 |
| Journal | Carcinogenesis | Volume | 20 |
| Issue | 2 | Pages | 325-32 |
| PubMed ID | 10069472 | Mgi Jnum | J:53702 |
| Mgi Id | MGI:1333304 | Doi | 10.1093/carcin/20.2.325 |
| Citation | Mohr U, et al. (1999) Possible carcinogenic effects of X-rays in a transgenerational study with CBA mice. Carcinogenesis 20(2):325-32 |
| abstractText | A lifetime experiment using 4279 CBA/J mice was carried out to investigate whether the pre-conceptual exposure of sperm cells to X-ray radiation or urethane would result in an increased cancer risk in the untreated progeny, and/or increased susceptibility to cancer following exposure to a promoting agent. The study consisted of four main groups, namely a control group (saline), a urethane group (1 mg/g body wt) and two X-ray radiation groups (1 Gy, 2 Gy), At 1, 3 and 9 weeks after treatment, the males of these four parental groups were mated with untreated virgin females. The offspring of each parental group was divided into two subgroups: one received s.c. Urethane (0.1 mg/g body wt once) as a promoter, the other saline, at the age of 6 weeks, All animals were evaluated for the occurrence of tumours, K-ras oncogene and p53 tumour suppressor gene mutations were investigated in frozen lung tumour samples. The female offspring of male parents exposed to X-rays 1 week before their mating showed a trend towards a higher tumour incidence of the haematopoietic system than the F1 controls. In addition, a higher percentage of bronchioloalveolar adenocarcinomas in male offspring born to irradiated paternals mated 1 week after X-ray treatment points to a plausible increased sensitivity of post- meiotic germ cell stages towards transgenerational carcinogenic effects, On the other hand, no increased tumour incidence and malignancy were observed in the offspring born to irradiated paternals mated 3 and 9 weeks after X-ray treatment. Paternal urethane treatment 1, 3 and 9 weeks prior to conception did not result in significantly altered incidence or malignancy of tumours of the lung, liver and haematopoietic tissue in the offspring. K-rns mutations increased during tumour progression from bronchioloalveolar hyperplasia to adenoma, Codon 61 K-ras mutations were more frequent in lung tumours of urethane-promoted progeny from irradiated parents than from control parents. P53 mutations were absent from these lung alterations. |
