| First Author | Jippo T | Year | 1999 |
| Journal | Blood | Volume | 93 |
| Issue | 6 | Pages | 1942-50 |
| PubMed ID | 10068667 | Mgi Jnum | J:53440 |
| Mgi Id | MGI:1332729 | Doi | 10.1182/blood.v93.6.1942.406k08_1942_1950 |
| Citation | Jippo T, et al. (1999) Deficient transcription of mouse mast cell protease 4 gene in mutant mice of mi/mi genotype. Blood 93(6):1942-50 |
| abstractText | The mi locus encodes a member of the basic-helix-loop- helix-leucine zipper (bHLH-Zip) protein family of transcription factors thereafter called MITF). We reported that expression of the mouse mast cell protease 5 (MMCP-5) and MMCP-6 genes were deficient in cultured mast cells (CMC) derived from mutant mice of mi/mi genotype. Despite the reduced expression of both MMCP-5 and MMCP-6, their regulation mechanisms were different. Because MMCP-5 is a chymase and MMCP-6 a tryptase, there was a possibility that the difference in regulation mechanisms was associated with their different characteristics as proteases. We compared the regulation mechanisms of another chymase, MMCP-4 with those of MMCP-5 and MMCP-6. The expression of the MMCP-4 gene was also deficient in mi/ mi CMC. The overexpression of the normal (+) MITF but not of mi-MITF normalized the poor expression of the MMCP-4 gene in mi/mi CMC, indicating the involvement of +-MITF in transactivation of the MMCP-4 gene. Although MMCP-4 is chymase as MMCP-5, the regulation of MMCP-4 expression was more similar to MMCP-6 than to MMCP-5. We also showed the deficient expression of granzyme B and cathepsin G genes in mi/mi CMC. Genes encoding granzyme B, cathepsin G, MMCP- 4 and MMCP-5 are located on chromosome 14. Because all these genes showed deficient expression in mi/mi CMC, there is a possibility that MITF might regulate the expression of these genes through a locus control region. (C) 1999 by The American Society of Hematology. |
