| First Author | Shimozato O | Year | 1999 |
| Journal | Biochem Biophys Res Commun | Volume | 256 |
| Issue | 3 | Pages | 519-26 |
| PubMed ID | 10080930 | Mgi Jnum | J:53972 |
| Mgi Id | MGI:1333698 | Doi | 10.1006/bbrc.1999.0336 |
| Citation | Shimozato O, et al. (1999) Expression of CD30 ligand (CD153) on murine activated T cells. Biochem Biophys Res Commun 256(3):519-26 |
| abstractText | CD30, a member of the TNF receptor family, has been implicated in the activation of T cells and B cells. In the present study, we characterized the expression and function of murine CD30 ligand (mCD153) by utilizing mCD153 transfectants and a novel mAb against mCD153 (RM153), which can inhibit the binding of murine CD30 to mCD153. The mCD153 transfectants did not co-stimulate the proliferation of anti-CD3-stimulated naive T cells but enhanced the proliferation of anti-CD28-co-stimulated T cells. The mCD153 transfectants exhibited a potent co-stimulatory activity for proliferation of pre-activated T cells that expressed CD30 after anti-CD3 and anti-CD28 stimulation. In contrast to the CD30 expression on naive T cells that required anti-CD28 co-stimulation, mCD153 expression was observed on anti-CD3-stimulated T cells without the anti-CD28 co-stimulation, predominantly on CD4(+) T cells with a transient kinetics which peaked at 24 h but disappeared at 48 h. In contrast to the preferential expression of CD30 on Th2 cells, mCD153 was expressed on both Th1 and Th2 cells after anti-CD3 stimulation. These results indicated a differential regulation of CD30 and CD153 expression in T cells, which may be relevant to immuno-regulatory role of the CD30-CD153 interaction. Copyright 1999 Academic Press. |
