| First Author | Giordano D | Year | 1999 |
| Journal | FEBS Lett | Volume | 446 |
| Issue | 2-3 | Pages | 218-22 |
| PubMed ID | 10100844 | Mgi Jnum | J:54264 |
| Mgi Id | MGI:1334865 | Doi | 10.1016/s0014-5793(99)00227-6 |
| Citation | Giordano D, et al. (1999) cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation. FEBS Lett 446(2-3):218-22 |
| abstractText | The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a approximately 9 kb transcript in bovine lung as well as in mouse neuroblastoma cells. Hybridization on total RNA extracted from dibutyryl-cAMP-treated NG108-15 cells shows a 5-fold increase of PDE5 9 kb mRNA: such an increase is not observed in N18TG2 although we observed a similar increase in the enzymatic activity of both cell lines. Our data demonstrate that PDE5 gene expression can be regulated by cAMP and suggest the existence of a complex regulatory system for PDE5 activity. |
