| First Author | Inoue S | Year | 1999 |
| Journal | Biochem Biophys Res Commun | Volume | 261 |
| Issue | 2 | Pages | 412-8 |
| PubMed ID | 10425199 | Mgi Jnum | J:56705 |
| Mgi Id | MGI:1342199 | Doi | 10.1006/bbrc.1999.0874 |
| Citation | Inoue S, et al. (1999) Molecular cloning of rat efp: expression and regulation in primary osteoblasts. Biochem Biophys Res Commun 261(2):412-8 |
| abstractText | We have previously identified an estrogen-responsive gene, efp (estrogen-responsive finger protein), by genomic binding-site cloning method. Here, we isolated a rat homologue of efp cDNA that encodes an open reading frame of 644 amino acids sharing high homology with human efp (69% identity at the protein level) and mouse efp (80% identity at the protein level). The efp protein has a RING finger, a variant type of zinc finger motif, B1 box and B2 box, each having a pair of zinc fingers, and coiled-coil domain, belonging to the RING finger-B box-Coiled Coil (RBCC) family. Several members of RBCC family including efp have characteristic C-terminal domain, forming a subfamily. Next, we detected efp mRNA in primary osteoblasts, one of estrogen target cells, derived from the calvariae of rat fetus. An anti-efp antibody revealed the efp protein is expressed and regulated by estrogen in the primary osteoblasts. Interestingly, the efp protein in primary osteoblasts is down-regulated by 1alpha,25-dihydroxyvitamin D(3) treatment that promotes the differentiation of the cells, whereas it is up-regulated by TGF-beta1 treatment that inhibits the differentiation of the cells. These findings suggest the possible involvement of the efp in the differentiation of osteoblastic cells. Copyright 1999 Academic Press. |
