| First Author | Yang D | Year | 1999 |
| Journal | Cell Immunol | Volume | 195 |
| Issue | 1 | Pages | 28-36 |
| PubMed ID | 10433794 | Mgi Jnum | J:56781 |
| Mgi Id | MGI:1342411 | Doi | 10.1006/cimm.1999.1517 |
| Citation | Yang D, et al. (1999) Cluster formation by protein kinase Ctheta during murine T cell activation: effect of age. Cell Immunol 195(1):28-36 |
| abstractText | Protein kinase Ctheta; (PKCtheta;) is thought to play an important role in T cell activation, in that exposure of cloned T cells to antigen-presenting cells bearing agonist peptides, but not antagonist peptides, leads to clustering of PKCtheta; molecules in the section of the T cell plasma membrane that is in contact with the APCs. To see whether aging affects this PKCtheta; clustering reaction in mouse T lymphocytes, we used immunofluorescence staining and confocal microscopy to observe the localization of PKCtheta; in CD4 and CD8 T lymphocytes activated by coincubation with anti-CD3 hybridoma cells. Aging led to a twofold decline in the proportion of both CD4 and CD8 T cells in which PKCtheta; underwent cluster formation. This decrease with age was not due to differences in the number of cell conjugates formed, nor to kinetic differences of PKCtheta; clustering, nor to the accumulation of memory T cells in old mice. There were no effects of aging on the levels or kinase activity of PKCtheta; in murine T cells. Our data suggest alterations in the upstream signals that regulate PKCtheta; translocation. Copyright 1999 Academic Press. |
