| First Author | Michikawa T | Year | 1999 |
| Journal | Neuron | Volume | 23 |
| Issue | 4 | Pages | 799-808 |
| PubMed ID | 10482245 | Mgi Jnum | J:57344 |
| Mgi Id | MGI:1344241 | Doi | 10.1016/s0896-6273(01)80037-4 |
| Citation | Michikawa T, et al. (1999) Calmodulin mediates calcium-dependent inactivation of the cerebellar type 1 inositol 1,4,5-trisphosphate receptor. Neuron 23(4):799-808 |
| abstractText | The dependency of purified mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP3R1)/Ca2+ channel function on cytoplasmic Ca2+ was examined. In contrast to the channels in crude systems, the purified IP3R1 reconstituted into planar lipid bilayers did not show the bell-shaped dependence on Ca2+. It was activated with increasing Ca2+ sublinearly without inhibition even up to 200 microM. The addition of calmodulin to the cytoplasmic side inhibited the channel at high Ca2+ concentrations. Calmodulin antagonists reversed the Ca2+-dependent inactivation of the native channels in cerebellar microsomes. These results indicate that the bell-shaped dependence on cytoplasmic Ca2+ is not an intrinsic property of the IP3R1, and the Ca2+-dependent inactivation is directly mediated by calmodulin. |
