| First Author | Guimond A | Year | 2002 |
| Journal | FEBS Lett | Volume | 515 |
| Issue | 1-3 | Pages | 13-9 |
| PubMed ID | 11943186 | Mgi Jnum | J:76002 |
| Mgi Id | MGI:2178199 | Doi | 10.1016/s0014-5793(02)02378-5 |
| Citation | Guimond A, et al. (2002) Site-directed mutagenesis of the type II TGF-beta receptor indicates a ligand-binding site distinct from that of the type II activin receptor. FEBS Lett 515(1-3):13-9 |
| abstractText | Site-directed mutagenesis was used to map the ligand-binding surface of the type II transforming growth factor-beta receptor extracellular domain (TbetaRII-ECD). Two putative ligand-binding sites were probed, the first being a predicted hydrophobic patch, the second being the finger 1 surface loop. Nine residues were mutated in the context of full-length TbetaRII and the effect of these mutations on ligand-binding and receptor signaling was analyzed. Complementary information was obtained by examining 'natural' evolutionary TbetaRII mutations. Together, the results indicate that residues within the finger 1 region, but not the hydrophobic patch, of the TbetaRII-ECD are required for productive ligand-binding. We conclude that, surprisingly, the ECDs of TbetaRII and type II activin receptor utilize distinct interacting surfaces for binding their respective ligands. |
