| First Author | Stewart M | Year | 2002 |
| Journal | J Virol | Volume | 76 |
| Issue | 9 | Pages | 4364-9 |
| PubMed ID | 11932403 | Mgi Jnum | J:75971 |
| Mgi Id | MGI:2178166 | Doi | 10.1128/JVI.76.9.4364-4369.2002 |
| Citation | Stewart M, et al. (2002) The common retroviral insertion locus dsi1 maps 30 kilobases upstream of the p1 promoter of the murine runx3/cbfa3/aml2 gene. J Virol 76(9):4364-9 |
| abstractText | The Dsi1 locus was identified as a common integration site for Moloney murine leukemia virus (MLV) in rat thymic lymphomas, but previous efforts to identify a gene affected by these insertions were unsuccessful. We considered the Runx3 gene a potential candidate on the basis of genetic mapping which showed that Dsi1 and Runx3 are closely linked on mouse chromosome 4 and the precedent of the related Runx2 gene, which emerged recently as a Myc-collaborating gene activated by retroviral insertion in thymic lymphomas of CD2-MYC mice. We now report the physical mapping of the Dsi1 locus to a site 30 kb upstream of the distal (P1) promoter of the murine Runx3 gene. Comparison with the syntenic region of human chromosome 1 shows that the next gene is over 250 kb 5' to Runx3, suggesting that Runx3 may be the primary target of retroviral insertions at Dsi1. Screening of CD2-MYC lymphomas for rearrangements at Dsi1 revealed a tumor cell line harboring an MLV provirus at this locus, in the orientation opposite that of Runx3. Proviral insertion was associated with very high levels of expression of Runx3, with a preponderance of transcripts arising at the P1 promoter. These results confirm that Runx3 is a target of retroviral insertions at Dsi1 and indicate that Runx3 can act as an alternative to Runx2 as a Myc-collaborating gene in thymic lymphoma. |
