| First Author | Gil D | Year | 2002 |
| Journal | Cell | Volume | 109 |
| Issue | 7 | Pages | 901-12 |
| PubMed ID | 12110186 | Mgi Jnum | J:77743 |
| Mgi Id | MGI:2182508 | Doi | 10.1016/s0092-8674(02)00799-7 |
| Citation | Gil D, et al. (2002) Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell 109(7):901-12 |
| abstractText | How membrane receptors initiate signal transduction upon ligand binding is a matter of intense scrutiny. The T cell receptor complex (TCR-CD3) is composed of TCR alpha/beta ligand binding subunits bound to the CD3 subunits responsible for signal transduction. Although it has long been speculated that TCR-CD3 may undergo a conformational change, confirmation is still lacking. We present strong evidence that ligand engagement of TCR-CD3 induces a conformational change that exposes a proline-rich sequence in CD3 epsilon and results in recruitment of the adaptor protein Nck. This occurs earlier than and independently of tyrosine kinase activation. Finally, by interfering with Nck-CD3 epsilon association in vivo, we demonstrate that TCR-CD3 recruitment of Nck is critical for maturation of the immune synapse and for T cell activation. |
