| First Author | Hara T | Year | 2002 |
| Journal | Gene | Volume | 291 |
| Issue | 1-2 | Pages | 11-6 |
| PubMed ID | 12095674 | Mgi Jnum | J:77898 |
| Mgi Id | MGI:2182874 | Doi | 10.1016/s0378-1119(02)00605-4 |
| Citation | Hara T, et al. (2002) Genomic organization and chromosomal localization of the mouse protein kinase Calpha gene. Gene 291(1-2):11-6 |
| abstractText | Protein kinase C (PKC) is a family of ten isoforms of phospholipid-dependent serine/threonine kinases, which participate in many cellular responses including cell growth, differentiation, and tumorigenesis. Of the isoforms, PKCalpha is distributed ubiquitously in almost all tissues and involved in various signal transductions. Furthermore, PKCalpha plays an important role in the growth and malignant progression of some tumor cell lines. Elucidating the roles of PKCalpha in vivo would lead to understanding of the mechanism of tumorigenesis and other biological functions. In this study, we isolated and characterized genomic DNA clones of the mouse PKCalpha gene (Prkca). The Prkca gene was a unigene consisting of 17 exons and spanning at least 116 kb. All the exon-intron boundaries followed the GT/AG rule. The genomic structure of PKCalpha was markedly conserved among the mouse, human, and fly. By radiation hybrid mapping, the Prkca gene was closely linked to sequence-tagged site marker D11Mit258 that locates 65.0 cM from the centromere of chromosome 11, and its transcription was towards the centromere. This study shows that generation of PKCalpha-mutant mice may reveal the PKCalpha function in vivo. |
