| First Author | Lin WC | Year | 2002 |
| Journal | DNA Cell Biol | Volume | 21 |
| Issue | 8 | Pages | 551-60 |
| PubMed ID | 12215258 | Mgi Jnum | J:78567 |
| Mgi Id | MGI:2385401 | Doi | 10.1089/104454902320308924 |
| Citation | Lin WC, et al. (2002) Transcriptional activation of C/EBPbeta gene by c-Jun and ATF2. DNA Cell Biol 21(8):551-60 |
| abstractText | C/EBPbeta is one of the key transcription factors responsible for the induction of a wide array of genes. Like many proto-oncogenes and transcription factors, transcription of C/EBPbeta gene can be induced by multiple extracellular signals. Using nuclear extracts from lipopolysaccharide (LPS)-stimulated mouse liver, five trans-acting factor-binding motifs, URE1 (-376 to -352), URE2 (-253 to -223), URE3 (-220 to -190), URE4 (-123 to -103), and URE5 (-72 to -45) were identified by DNAse I footprinting assays. Competition and supershift analysis of the complexes formed at the URE2 and URE4 indicated that they contain CREB/ATF and AP-1 family factors. Furthermore, recombinant ATF2 and c-Jun proteins from mammalian and bacterial cells can bind to URE2 and URE4 but not URE1. Cotransfection experiments showed that ATF2 and c-Jun activate the C/EBPbeta gene expression cooperatively through URE2 and URE4, and this activation was greatly increased under the treatment of low concentration of anisomycin. During acute phase response, the phosphorylation of c-Jun and ATF2 was found to correlate with C/EBPbeta gene expression. Taken together, our results provide the evidences that both c-Jun and ATF2 are the regulators of C/EBPbeta gene. |
