| First Author | Supattapone S | Year | 2001 |
| Journal | J Virol | Volume | 75 |
| Issue | 3 | Pages | 1408-13 |
| PubMed ID | 11152514 | Mgi Jnum | J:79419 |
| Mgi Id | MGI:2388202 | Doi | 10.1128/JVI.75.3.1408-1413.2001 |
| Citation | Supattapone S, et al. (2001) Identification of two prion protein regions that modify scrapie incubation time. J Virol 75(3):1408-13 |
| abstractText | A series of prion transmission experiments was performed in transgenic (Tg) mice expressing either wild-type, chimeric, or truncated prion protein (PrP) molecules. Following inoculation with Rocky Mountain Laboratory (RML) murine prions, scrapie incubation times for Tg(MoPrP)4053, Tg(MHM2)294/Prnp(0/0), and Tg(MoPrP, Delta23-88)9949/Prnp(0/0) mice were approximately 50, 120, and 160 days, respectively. Similar scrapie incubation times were obtained after inoculation of these lines of Tg mice with either MHM2(MHM2(RML)) or MoPrP(Delta23-88)(RML) prions, excluding the possibility that sequence-dependent transmission barriers could account for the observed differences. Tg(MHM2)294/Prnp(0/0) mice displayed prolonged scrapie incubation times with four different strains of murine prions. These data provide evidence that the N terminus of MoPrP and the chimeric region of MHM2 PrP (residues 108 through 111) both influence the inherent efficiency of prion propagation. |
