| First Author | Aifantis I | Year | 2002 |
| Journal | Nat Immunol | Volume | 3 |
| Issue | 5 | Pages | 483-8 |
| PubMed ID | 11927911 | Mgi Jnum | J:76266 |
| Mgi Id | MGI:2178925 | Doi | 10.1038/ni779 |
| Citation | Aifantis I, et al. (2002) A critical role for the cytoplasmic tail of pTalpha in T lymphocyte development. Nat Immunol 3(5):483-8 |
| abstractText | Signals that emanate from the pre-T cell receptor (pre-TCR) regulate multiple processes required for the development of the alphabeta T cell lineage. In contrast to the gammadelta TCR, the pre-TCR localizes cell-autonomously to membrane rafts, where it appears to signal in a constitutive and ligand-independent manner. We addressed here the role played by structural features specific to the cytoplasmic domain of the pre-TCRalpha chain (pTalpha). More specifically, we examined a COOH-terminal proline-rich sequence that might play a role in signal transduction and a juxtamembrane cysteine residue that could be a target for palmitoylation, thus allowing spontaneous raft localization. Expression of pTalpha mutants in transgenic mice, retrovirally transduced T cell precursors and cell lines showed that the pTalpha cytoplasmic tail, in particular the proline-rich domain, plays a crucial role in pre-TCR signaling and T cell development. In contrast, the pTalpha juxtamembrane cysteine appeared to be dispensable for pre-TCR function. |
