| First Author | Takechi S | Year | 2002 |
| Journal | Biochim Biophys Acta | Volume | 1577 |
| Issue | 3 | Pages | 466-70 |
| PubMed ID | 12359338 | Mgi Jnum | J:79808 |
| Mgi Id | MGI:2388959 | Doi | 10.1016/s0167-4781(02)00463-3 |
| Citation | Takechi S, et al. (2002) Cloning and characterization of the chick Oct binding factor OBF-1. Biochim Biophys Acta 1577(3):466 |
| abstractText | We have cloned the chicken homolog of OBF-1, chOBF-1, which comprises 256 amino acids, and exhibits only 65% overall identity to the human and mouse OBF-1 proteins. Amino acid sequence alignment revealed the putative Oct-binding sequence, RPYQGVRVKEPVKELL(K/R)RKRG, which is conserved among chicken, mouse and human. chOBF-1 protein was demonstrated to bind chicken Oct-1 protein by the in vitro immunoprecipitation experiment, and chOBF-1 was shown to functionally activate the chicken immunoglobulin (Ig) light chain promoter in the NIH 3T3 cell. Taken together, these data indicate that the Ig gene transcription machinery, including Oct-1 and OBF-1, has been highly conserved in vertebrate evolution. |
