| First Author | Todt JC | Year | 2002 |
| Journal | J Biol Chem | Volume | 277 |
| Issue | 39 | Pages | 35906-14 |
| PubMed ID | 12114511 | Mgi Jnum | J:79313 |
| Mgi Id | MGI:2387792 | Doi | 10.1074/jbc.M202967200 |
| Citation | Todt JC, et al. (2002) Activation of protein kinase C beta II by the stereo-specific phosphatidylserine receptor is required for phagocytosis of apoptotic thymocytes by resident murine tissue macrophages. J Biol Chem 277(39):35906-14 |
| abstractText | We showed previously that protein kinase C (PKC) is required for phagocytosis of apoptotic leukocytes by murine alveolar (AMo) and peritoneal macrophages (PMo) and that such phagocytosis is markedly lower in AMo compared with PMo. In this study, we examined the roles of individual PKC isoforms in phagocytosis of apoptotic thymocytes by these two Mo populations. By immunoblotting, AMo expressed equivalent PKC eta but lower amounts of other isoforms (alpha, betaI, betaII, delta, epsilon, mu, and zeta), with the greatest difference in betaII expression. A requirement for PKC betaII for phagocytosis was demonstrated collectively by phorbol 12-myristate 13-acetate-induced depletion of PKC betaII, by dose-response to PKC inhibitor Ro-32-0432, and by use of PKC betaII myristoylated peptide as a blocker. Exposure of PMo to phosphatidylserine (PS) liposomes specifically induced translocation of PKC betaII and other isoforms to membranes and cytoskeleton. Both AMo and PMo expressed functional PS receptor, blockade of which inhibited PKC betaII translocation. Our results indicate that murine tissue Mo require PKC betaII for phagocytosis of apoptotic cells, which differs from the PKC isoform requirement previously described in Mo phagocytosis of other particles, and imply that a crucial action of the PS receptor in this process is PKC betaII activation. |
