| First Author | Hambrock HO | Year | 2003 |
| Journal | J Biol Chem | Volume | 278 |
| Issue | 13 | Pages | 11351-8 |
| PubMed ID | 12538579 | Mgi Jnum | J:82580 |
| Mgi Id | MGI:2653715 | Doi | 10.1074/jbc.M212291200 |
| Citation | Hambrock HO, et al. (2003) SC1/hevin. An extracellular calcium-modulated protein that binds collagen I. J Biol Chem 278(13):11351-8 |
| abstractText | SC1, a member of the BM-40 family of extracellular matrix proteins, was recombinantly expressed in a eukaryotic expression system. The full-length protein as well as truncated versions were purified to homogeneity under non-denaturing conditions. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of full-length SC1 revealed a mass of 87.8 kDa of which 16.8 kDa is contributed by posttranslational modifications. In electron microscopy, after negative staining, SC1 was revealed as a globule attached to a thread-like structure. A calcium dependence of the SC1 conformation could be demonstrated by fluorescence spectroscopy. In the extracellular matrix of cultured osteosarcoma cells SC1 was found associated with collagen I-containing fibrils, and binding of SC1 to reconstituted collagen I fibrils could be demonstrated by immunogold labeling and electron microscopy. SC1 showed a broad expression in a variety of tissues. |
