| First Author | Figueroa A | Year | 2003 |
| Journal | Mol Cell Biol | Volume | 23 |
| Issue | 14 | Pages | 4991-5004 |
| PubMed ID | 12832484 | Mgi Jnum | J:84385 |
| Mgi Id | MGI:2667514 | Doi | 10.1128/MCB.23.14.4991-5004.2003 |
| Citation | Figueroa A, et al. (2003) Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes. Mol Cell Biol 23(14):4991-5004 |
| abstractText | In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes. |
