| First Author | Shinagawa T | Year | 2003 |
| Journal | Genes Dev | Volume | 17 |
| Issue | 11 | Pages | 1340-5 |
| PubMed ID | 12782652 | Mgi Jnum | J:83742 |
| Mgi Id | MGI:2663380 | Doi | 10.1101/gad.1073003 |
| Citation | Shinagawa T, et al. (2003) Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter. Genes Dev 17(11):1340-5 |
| abstractText | We have developed a new vector, named pDECAP, to express long double-strand RNA (ds-RNA) from an RNA polymerase II (Pol II) promoter. Because the transcripts from pDECAP lack both the 5'-cap structure and the 3'-poly(A) tail that facilitate ds-RNA export to the cytoplasm, long ds-RNA from pDECAP does not induce the interferon response. Transgenic mice embryos expressing long ds-RNA for the transcriptional corepressor Ski from this vector exhibited phenotypes that were remarkably similar to those of Ski-deficient embryos, including defects of neural tube closure and eye formation. Thus, this vector provides a new tool to efficiently generate tissue-specific knockdown mice for studying gene function in whole animal systems. |
