| First Author | Tchernychev B | Year | 2003 |
| Journal | J Cell Biol | Volume | 163 |
| Issue | 5 | Pages | 1145-55 |
| PubMed ID | 14662752 | Mgi Jnum | J:87184 |
| Mgi Id | MGI:2683834 | Doi | 10.1083/jcb.200310079 |
| Citation | Tchernychev B, et al. (2003) Peritoneal macrophages express both P-selectin and PSGL-1. J Cell Biol 163(5):1145-55 |
| abstractText | Macrophages, phagocytic cells involved in an early phase of host defense, are known to express the P-selectin ligand, PSGL-1. Heretofore, P-selectin has only been found on platelets and endothelial cells. Here, we demonstrate that peritoneal macrophages isolated by peritoneal lavage of unchallenged mice express P-selectin on the plasma membrane. The peritoneal macrophages synthesize P-selectin, as indicated by metabolic labeling experiments. P-Selectin is constitutively expressed on the extracellular surface of macrophages but is only partially colocalized with PSGL-1. P-Selectin is rapidly translocated from the macrophage plasma membrane to intracellular vesicles and to lysosomes. Peritoneal macrophages assemble into cell strings under flow conditions based upon macrophage-macrophage interactions mediated by P-selectin and PSGL-1. This is the first description of a leukocyte shown to express both P-selectin and PSGL-1. |
