| First Author | Dasgupta T | Year | 2004 |
| Journal | J Ethnopharmacol | Volume | 92 |
| Issue | 1 | Pages | 23-36 |
| PubMed ID | 15099843 | Mgi Jnum | J:90180 |
| Mgi Id | MGI:3042660 | Doi | 10.1016/j.jep.2003.12.004 |
| Citation | Dasgupta T, et al. (2004) Chemopreventive potential of Azadirachta indica (Neem) leaf extract in murine carcinogenesis model systems. J Ethnopharmacol 92(1):23-36 |
| abstractText | Numerous laboratory studies reveal that various naturally occurring dietary substances can modify the patho-physiological process of various metabolic disorders and can be an effective preventive strategy for various diseases, including cancer. Indian Neem tree, Azadirachta indica A. Juss. (family: Meliaceae), contains at least 35 biologically active principles and is widely grown all over the tropics. The effect of two different doses (250 and 500mg per kilogram body weight) of 80% ethanolic extract of the leaves of Azadirachta indica were examined on drug metabolizing Phase-I and Phase-II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase, and lipid peroxidation in the liver of 7-week-old Swiss albino mice. Also anticarcinogenic potential of Azadirachta indica leaf extract was studied adopting protocol of benzo(a)pyrene-induced fore-stomach and 7,12-dimethyl benz(a)anthracene (DMBA)-induced skin papillomagenesis. Our primary findings reveal its potential to induce only the Phase-II enzyme activity associated mainly with carcinogen detoxification in liver of mice. The hepatic glutathione S-transferase ( [Formula: see text] ) and DT-diaphorase specific activities ( [Formula: see text] ) were elevated above basal level. With reference to antioxidant enzymes the investigated doses were effective in increasing the hepatic glutathione reductase (GR), glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) activities significantly (from [Formula: see text] to [Formula: see text] ). Reduced glutathione measured as non-protein sulphydryl was found to be significantly elevated in liver ( [Formula: see text] ) and in extrahepatic organs (from [Formula: see text] to [Formula: see text] ) examined in our study. Glutathione S-transferase (GST) and DT-diaphorase (DTD) showed a dose-dependent increase in extrahepatic organs. Chemopreventive response was measured by the average number of papillomas per mouse, as well as percentage of tumor-bearing animals. There was a significant inhibition of tumor burden, in both the tumor model system studied (from [Formula: see text] to [Formula: see text] ). Tumor incidence was also reduced by both the doses of Azadirachta indica extract. |
