| First Author | Cabarrocas J | Year | 2004 |
| Journal | J Autoimmun | Volume | 22 |
| Issue | 3 | Pages | 179-89 |
| PubMed ID | 15041038 | Mgi Jnum | J:90579 |
| Mgi Id | MGI:3044248 | Doi | 10.1016/j.jaut.2004.01.001 |
| Citation | Cabarrocas J, et al. (2004) A transgenic mouse model for T-cell ignorance of a glial autoantigen. J Autoimmun 22(3):179-89 |
| abstractText | The fate of autoreactive CD4+T cells was investigated in HNT-TCR x GFAP-HA double transgenic mice, in which the majority of CD4+T cells is specific for a neo-selfantigen expressed under a glial cell-specific promoter. These mice do not develop any clinical or histological signs of central or enteric nervous system autoimmunity. Although HA is transcribed in the thymus of GFAP-HA mice, similar numbers of CD4+ CD8- thymocytes, expressing comparable levels of the transgenic TCR, developed in HNT-TCR x GFAP-HA double transgenic and HNT-TCR single transgenic mice, indicating that HA-specific thymocytes are not negatively selected. In the periphery, the HA-specific T cells remained similarly unaffected as they displayed a naive phenotype and were neither deleted nor anergized. Finally, immunization of HNT-TCR x GFAP-HA mice with the HNT peptide in CFA and/or in vivo depletion of CD25+ cells did not reverse this state of immune ignorance as judged by the lack of clinical manifestations of intestinal and neurological disease in these mice. Taken together these data demonstrate a profound state of immune ignorance towards a self-antigen expressed in the enteric and central nervous system. |
