| First Author | Park KC | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 323 |
| Issue | 3 | Pages | 1055-62 |
| PubMed ID | 15381106 | Mgi Jnum | J:92824 |
| Mgi Id | MGI:3054583 | Doi | 10.1016/j.bbrc.2004.08.204 |
| Citation | Park KC, et al. (2004) Transglutaminase 2 induces nitric oxide synthesis in BV-2 microglia. Biochem Biophys Res Commun 323(3):1055-62 |
| abstractText | A hallmark of brain inflammation is the activation of microglia. Excessive production of nitric oxide (NO), as a consequence of increased inducible nitric oxide synthase (iNOS) in glia, contributes to neurodegeneration. Transglutaminase 2 (TGase 2) is a cross-linking enzyme, which is increased in neurodegeneration. TGase 2 is also considered to be a useful and reliable marker for activation levels in resident and inflammatory macrophages. Therefore, an increase of TGase 2 expression may contribute to activation of microglia. To test this hypothesis, we analyzed the expression of TGase 2 in BV-2 microglia activated with lipopolysaccharide (LPS). Total TGase activity was increased about 5-fold after 24h exposure to LPS. The increase of NO synthesis is correlated with increase of TGase 2 expression. Secretion of NO was reduced between 40 and 80% by TGase inhibition in a dose-dependent manner. This suggests that TGase 2 appears to control iNOS transcription. |
