| First Author | Masuda A | Year | 2004 |
| Journal | J Immunol | Volume | 173 |
| Issue | 9 | Pages | 5564-73 |
| PubMed ID | 15494506 | Mgi Jnum | J:93741 |
| Mgi Id | MGI:3505637 | Doi | 10.4049/jimmunol.173.9.5564 |
| Citation | Masuda A, et al. (2004) The interaction between GATA proteins and activator protein-1 promotes the transcription of IL-13 in mast cells. J Immunol 173(9):5564-73 |
| abstractText | IL-13 is considered to be a key modulator in the pathogenesis of Th2-induced allergic inflammation, although little is known about the regulation of IL-13 transcription in mast cells. In T cells, involvement of GATA-3 in cell type-specific expression of the IL-13 gene has been reported. However, the mechanisms that induce rapid transactivation of the IL-13 gene in response to various types of stimulation have hitherto remained unknown. In this report, we describe our investigation of the promoter region necessary for IL-13 transcription; we have found that both AP-1 and GATA proteins are indispensable for IL-13 transcription in mouse mast cells. In our investigation, we focused on the functional interaction between GATA and AP-1 in the IL-13 promoter context. Transfection experiments have revealed that GATA-1 and GATA-2 proteins are able to associate with AP-1 proteins. We have also shown that overexpression of GATA-1 induced excess AP-1 binding to the IL-13 promoter as well as a significant increase in IL-13 production in mast cells. The results of the present study have shown that direct interaction between AP-1 and GATA proteins plays an important role in IL-13 transcription in mast cells. |
