| First Author | Chang HY | Year | 2004 |
| Journal | J Cell Sci | Volume | 117 |
| Issue | Pt 26 | Pages | 6289-96 |
| PubMed ID | 15561765 | Mgi Jnum | J:94442 |
| Mgi Id | MGI:3512818 | Doi | 10.1242/jcs.01567 |
| Citation | Chang HY, et al. (2004) Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggs. J Cell Sci 117(Pt 26):6289-96 |
| abstractText | Metaphase II-arrested mouse eggs are stimulated to complete meiosis by sperm-induced Ca(2+) spiking. The Ca(2+) signal causes activation of the E3 ligase anaphase-promoting complex/cyclosome (APC), leading to the destruction of key proteins necessary for meiotic exit. We show, using western blots of mouse eggs, the presence of both APC activators cdc20 and cdh1, which target D-box and D-box/KEN-box substrates, respectively, for proteolysis. We decided to examine the temporal activation of APC(cdc20) and APC(cdh1) by coupling APC substrates to GFP and examining their destruction in real-time following release from second meiotic division arrest. D-box substrates were degraded quickly after the initiation of sperm-induced Ca(2+) spiking, such that their degradation was complete by the time of second polar body extrusion. By contrast, KEN-box-containing substrates were degraded when CDK1 activity was low, during the period between polar body extrusion and pronucleus formation. This observation of apparent APC(cdh1) activity in meiosis II based on destruction of exogenous GFP-coupled substrates was then confirmed by observing destruction of endogenous APC(cdh1) substrates. These data are consistent with a model of initial APC(cdc20) activation on sperm-induced activation, followed by APC(cdh1) activation after second polar body extrusion. Interestingly, therefore, we propose that mammalian eggs undergo meiosis II with both APC(cdc20) and APC(cdh1), whereas eggs of other species so far described have APC(cdc20) activity only. |
