| First Author | Iwaki D | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 328 |
| Issue | 1 | Pages | 173-6 |
| PubMed ID | 15670766 | Mgi Jnum | J:95438 |
| Mgi Id | MGI:3525987 | Doi | 10.1016/j.bbrc.2004.12.162 |
| Citation | Iwaki D, et al. (2005) The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain. Biochem Biophys Res Commun 328(1):173-6 |
| abstractText | CD14 has been shown to enhance Toll-like receptor 2 (TLR2)-mediated signaling in response to peptidoglycan. Anti-CD14 monoclonal antibody MEM-18, whose epitope was located at the amino acid residues 57-64, blocked the binding of sCD14 to the recombinant soluble form of the extracellular TLR2 domain (sTLR2). The deletion mutant sCD14(Delta57-64) lacking the amino acid residues 57-64 failed to bind to sTLR2. Cotransfection of wild type mCD14 but not mCD14(Delta57-64) with TLR2 enhanced NF-kappaB activation in response to peptidoglycan. These results indicate that the CD14 region spanning amino acids 57-64 is critical for interacting with TLR2 and enhancing TLR2-mediated peptidoglycan signaling. |
