| First Author | Gong LW | Year | 2005 |
| Journal | Proc Natl Acad Sci U S A | Volume | 102 |
| Issue | 14 | Pages | 5204-9 |
| PubMed ID | 15793002 | Mgi Jnum | J:97403 |
| Mgi Id | MGI:3575375 | Doi | 10.1073/pnas.0501412102 |
| Citation | Gong LW, et al. (2005) Phosphatidylinositol phosphate kinase type I{gamma} regulates dynamics of large dense-core vesicle fusion. Proc Natl Acad Sci U S A 102(14):5204-9 |
| abstractText | Phosphatidylinositol-4,5-bisphosphate was proposed to be an important regulator of large dense-core vesicle exocytosis from neuroendocrine tissues. Here, we have examined the kinetics of secretion in chromaffin cells from mice lacking phosphatidylinositol phosphate kinase type Igamma, the major neuronal phosphatidylinositol-4-phosphate 5-kinase. Absence of this enzyme caused a reduction of the readily releasable vesicle pool and its refilling rate, with a small increase in morphologically docked vesicles, indicating a defect in vesicle priming. Furthermore, amperometry revealed a delay in fusion pore expansion. These results provide direct genetic evidence for a key role of phosphatidylinositol-4,5-bisphosphate synthesis in the regulation of large dense-core vesicle fusion dynamics. |
